Cell Transfection Reagents

Epoch Life Science"s GenCarrier ^TM transfection reagents apply a proprietary Lipoplex-enhancing mechanism to obtain the best transfection outcome - highest transgene expression and lowest cytotoxicity.

Epoch Life Science is determined to maintain the highest standards and deliver the highestquality products and services. Every batch is strictly tested to meet the requirementsof cell-culture grade bioproducts.

GenCarrier-1^TM DNA transfection reagent - works in serum containing media

GenCarrier-1^TM has been used successfully to transfect DNA into HeLa, CHO-K1, COS, LNCaP,NIH3T3, U937, SF-9, S2 (Schneider - Drosophila), 293, T-24, C2C12, PANC-1, primary human keratinocytes,primary aortic smooth muscle, primary rabbit myoblasts, human bone marrow endothelial cells (HBMEC),and other cell lines. No significant toxicity was observed at working concentrations on the cell lines tested.

Enough reagent is provided for approximately 250 transfections in 35-mm plates per package.

PC12 cells transfected withpCMV-GFP using GenCarrier-1(upper 2 pictures) and lipofectamine 2000 (lower 2 pictures)

GenCarrier-2^TM DNA transfection reagent -works in primary cell culture GenCarrier-2^TM has been used successfully to transfect DNA into COS, HepG2, PC12, NIH3T3, Jurkat, PC-12, HUVEC, primary human pancreatic islet cells, primary rat and hamsterhepatocytes, primary rat cardiomyocytes, primary male rat germ cells, primary chick retinal neurons,primary rat fibroblasts, primary human keratinocytes, and tracheobroncial cells. No significant toxicitywas observed at working concentrations on the cell lines tested.

Enough reagent is provided for approximately 250 transfections in 35-mm plates per package.

Human primary pancreatic cells transfected with pCMV-bGal using GenCarrier-2 showed >50% transfection efficiency

RNAiCarrier^TM siRNA transfection reagent

RNAiCarrier^TM has been used successfully to transfect siRNA into HEK293, HuH-7 (human hepatoma), B16 melanoma, CHO-K1, COS-1, COS-7, NIH3T3, SF-9, S2, primary human keratinocytes, primary rat aortic smooth muscle (WKY3M), and other cell lines. No significant toxicity was observed at working concentrations used on the cell lines tested.

Enough reagent is provided for approximately 250 transfections in 35-mm plates per package.

在Epoch Life Science，已经研究了具有不同成分的二氧化硅膜的核酸结合特性。仔细定量和优化了在不同化学条件下（例如宽范围的pH值，离子强度，温度和生物学环境）的结合能力。我们的旋转柱仅由硅胶膜制成，该硅胶膜（a）选择性最强地结合核酸，（b）具有高容量，并且（c）容易释放结合的核酸，以提供优异的纯度和总体最高的回收率。