GenCatch TM Plasmid DNA Mini-Prep Kit
| Catalog# | size | Price | Quantity | |
|---|---|---|---|---|
| 2160050 | 50 Mini Preps | $45.00 | ||
| 2160250 | 250 Mini Preps | $185.00 |
Description | GenCatch TM Plasmid DNAMini Preparation provides a simple, fast and cost-effectivemethod to purify plasmid DNA without phenol/chloroformextraction. It is based on binding of DNA to silica-basedmembranes in a chaotropic salt. An average yield of 1-20 ugplasmid DNA can be expected from 1-5 ml overnight bacterialculture. | ||||
Detailed Components | High quality spin columns, collection tubes together with buffers and protocols. | ||||
Features | Fast: Up to 24 samples can be readily prepared in 30 minutes with minimum efforts. Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold. Convenient: No phenol extractions or ethanol precipitations required. Yield:4-20μg of DNA from 2ml of culture Quality DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing. | ||||
Technical Data |
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Plasmid DNA ranging from 4 to 12 kb purified from E. coli DH5a with GenCatch Plasmid DNA Miniprep Kit and run on 1% agarose gel. 
Restriction enzyme digestion of pBluescriptSK plasmid purified by GenCatchPlasmid DNA Miniprep Kit and analyzed on a 1.5% agarose gel.
Sequencing result of plasmid pBluescriptSK purified by GenCatchPlasmid DNA Miniprep Kit using ABI 3730xl sequencer. 在Epoch Life Science，已经研究了具有不同成分的二氧化硅膜的核酸结合特性。仔细定量和优化了在不同化学条件下（例如宽范围的pH值，离子强度，温度和生物学环境）的结合能力。我们的旋转柱仅由硅胶膜制成，该硅胶膜（a）选择性最强地结合核酸，（b）具有高容量，并且（c）容易释放结合的核酸，以提供优异的纯度和总体最高的回收率。
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