GenCatch TM PCR Cleanup Kit
| Catalog# | size | Price | Quantity | |
|---|---|---|---|---|
| 2360050 | 50 Preps | $45.00 | ||
| 2360250 | 250 Preps | $185.00 |
Description | GenCatch TM PCR Cleanup Kit purifies DNA molecules from PCR and other enzymatic reactions from enzymes, dNTPs, salts, and primers without phenol/chloroform extraction. Recovery efficiency of DNA fragments ranging from 70 bp to 10 kb in size is up to95%. It totally eliminate slurry carryover problem as encountered using silica beadsor glass milk based methods. Up to 10 ¦Ìg DNA can be recovered with a single columnand elution volumes can be as small as 10 ¦Ìl. | ||||
Detailed Components | High capacity/quality columns with buffers and protocols. | ||||
Features | Capacity: Up to 10 ¦Ìg of DNA. Elution Volume: 10-50 ¦Ìl . Recover Efficiency:Up to 95%. Quality: DNA purifiedusing GenCatch TM PCR Cleanup Kit is free from enzyme, polysaccharides, agraose,carry-over slurry and salt contamination. And is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformationand transfection, radioactive and fluorescent sequencing. Fast: Single samplescan be prepared within 10 minutes with minimum efforts. 18-24 samples canbe simultaneously processed in a centrifugation format within 30 minutes. Or, morecan be processed using a vacuum manifold. Flexible: Mini columnscan used in either centrifugation procedure or using a vacuum manifold. Convenient: No phenolextractions or ethanol precipitations required. | ||||
Technical Data |  PCR products of various MW (lane 1-5) were purified using GenCatch TM PCRCleanup Kit and loaded as a cocktail (lane 6) onto 1.5% agraose gel. Recovery efficiencycan be roughly estimated by comparing the overall intensity of the individual bands. | ||||
在Epoch Life Science，已经研究了具有不同成分的二氧化硅膜的核酸结合特性。仔细定量和优化了在不同化学条件下（例如宽范围的pH值，离子强度，温度和生物学环境）的结合能力。我们的旋转柱仅由硅胶膜制成，该硅胶膜（a）选择性最强地结合核酸，（b）具有高容量，并且（c）容易释放结合的核酸，以提供优异的纯度和总体最高的回收率。
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