Description | | GenCatch TM Total RNA Extraction System provides an GenCatchmical method to purifytotal RNA from various samples such as cultured cells, tissues, bacteria, and yeast.A simple silica-membrane spin-column method can isolate total RNA without phenol/chloroformextraction and ethanol precipitation. Total RNA with a molecular size greater than200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) canbe isolated. |
Detailed Components | | High quality spin columns, collection tubes together with buffers and protocols. | |
Features | | | Miniprep | Midiprep | Maxiprep | Sample Size | | | | Tissue | 10-30 mg | 100-200 mg | 0.5-1 g | Cell culture | up to 1x 107 cells | up to 3-7x 107 cells | up to 2-10x 108 cells | Bacteria | up to 1x 109 cells | up to 1x 1010 cells | up to 5x 1010 cells | Yeast | up to 5x 107 cells | up to 5x 108 cells | up to 2x 109 cells | Yield | | | | Tissue | up to 45 ug | up to 450 ug | up to 2.5 mg | Cell culture | up to 30 ug | up to 300 ug | up to 6 mg | Bacteria/yeast | up to 65 ug | up to 650 ug | up to 3 mg | Elution Volume | 200 ul | 500 ul | 2.5 ml |
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Technical Data | | Total RNA isolated from tissue and culturedcells using GenCatch TM Total RNA Extraction System was analyzed by formaldehydeagarose gel electrophoresis. RT-PCR of total RNA extracted from Hela cellswith GenCatch TM Total RNA Extraction System. Lane 1-5 are RT-PCR productsof the total RNA. Lane 6-10 are RT-PCR of total RNA extracted from 106,105, 104, 103, and 102 HeLa cells respectively. |