Description | | GenCatch TM Plant RNA Purification System provides asimple,fast and cost-effective method to purify RNA Plant and fugal samples. Itcomes with shearing tubes for simple and fast homogenization and filtration of planttissues. It uses a simple silica-membrane spin-column method to isolate total RNAgreater than 200 nucleotides while excluding smaller RNAsuch as 5S, 5.8S and tRNAwithout phenol/chloroform extraction and ethanol precipitation. The system alsoprovides additional buffer for handling plant tissue that contai sticky secondarymetabolites. RNA isolated can be used for a variety of molecular manipulations suchas: Northern blotting, RT-PCR, PolyA+ RNA selection, cDNA synthesis,Primer extension and in vitro translation, etc. |
Detailed Components | | High quality spin columns, collection tubes together with buffers and protocols. | |
Features | | | Miniprep | Maxiprep | Sample Size | up tp 100 mg plant tissue | up to 1 g of plant tissue | Binding Capacity | up tp 100 ug | up to 1 mg | Elution Volume | 50 ul | 500 ul |
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Technical Data | | Formaldehyde agarose gel analysis of totalRNA isolated from various plant samples with GenCatch TM Plant RNA PurificationSystem. Lane 1: Tobacco; 2: Arabidopsis; 3: Rice; 4: Tomato leaves; 5: Tomato stem;6: Tomato root. 2 ug of total RNA was loaded per lane. RT-PCR if total RNA extracted from differenttissue of tomato with GenCatch TM Plant RNA Purification System. Lane 1: totalRNA from leaves; 2: total RNA from stem; 3: total RNA from root; 1 kb DNA markeris on the left. |